ALKALINE PHOSPHATASE AND ACID PHOSPHATASE LEVELS IN THE ABDOMINAL MUSCLES OF IMMUNOSTIMULATED MICE DURING HEPATITIS B

Abstract

The investigations were conducted in 8 groups of male Swiss Albino mice (6-8 wks old; 23-26 g wt). In mice of group I (10), Immunex DS (IDS) was orally administered (@150mg/mouse) as a single dose. In mice (10) of group A, B, C, D, E and F, IDS was given orally @150mg/mouse (single dose) (on day 0) and Gene Vac B vaccine was infected on day 4 of experiment @ 0.07ml/mouse, 0.1ml/mouse, 0.2ml/mouse 0.4ml/mouse, 0.8ml/mouse and 1ml/mouse. Another group (U) of mice (10) was kept untreated (with IDS and uninfected) as controls for comparison. Two mice from each experimental (A, B, C, D, E, and F) and control groups (U) were necropsied after day 7 of vaccine treatment. Abdominal muscle tissue was separated and analysed for total carbohydrate and total glycogen using standard methods. The level of total carbohydrate increased and glycogen decreased markedly from day 1 to 5 of experiment in group A. Mice of group B showed a decrease in the content of carbohydrate and glycogen on day 1 to 2 and a gradual increase from day 2 to 5 of experiment. In mice of group C, the level of carbohydrate decreased on day 1 and 3 and glycogen on day 1 and 2 with an increased content of carbohydrate and glycogen on day 2, 4, 5 and 3, 4 and 5. The content of carbohydrate and glycogen decreased on day 1, 2, 3 and increased on day 4, 5 in mice of group D. Mice of group E decreased level of carbohydrate on day 1, 3 and 4 and glycogen on day 1, 2 and 3 and an increased level of carbohydrate and glycogen on day 2 and 5 and 4 and 5. Mice of group F showed a gradual decrease in the level of carbohydrate from day 1 to 5 and and glycogen on day 1, 2 increased level of glycogen from day 3, 4 and 5 when compared with the control and immunostimulated mice group U and I. The altered level of carbohydrate and glycogen in all the experimental groups might be due to their disturbance in their metabolism.