Alkaline phophatase from the hepatopancreas of shrimp ( Pandalus borealis): A dimeric enzyme with catalytically active subunits

Abstract

1. 1. Alkaline phosphatase was purified to homogeneity from the cold water shrimp, Pandalus borealis. 2. 2. The enzyme is a dimeric protein with a mol. wt of 155,000. 3. 3. Contrary to other dimeric alkaline phosphatases the shrimp enzyme may be dissociated into subunits which retain enzyme activity. 4. 4. The shrimp alkaline phosphatase is completely inhibited by EDTA, but the activity can be restored to a large degree by zinc. 5. 5. The enzyme shows high stability towards SDS and 2-mercaptoethanol. 6. 6. The activity is moderately inhibited to the same extent by l-phenylalanine and l-homoarginine.