Abstract
An alkaline inorganic pyrophosphatase (IP) from leaves of an orchid, Aranda Christine 130 ( Arachnis hookerana var. luteola × Vanda Hilo Blue) was purified by acetone precipitation and chromatography on Sephadex G-75 and DEAE-cellulose. The IP gave a single band on non-denaturing gel electrophoresis at pH 8.3 and its M, determined by gel filtration, was 28 000. The pH optimum was 9 and the IP required Mg 2+ for its activity and stability. The IP exhibited high specificity for PPi and attained a maximum activity at a Mg 2+: PPi ratio of 10:1. Other cations tested could not replace Mg 2+ and they were also found to be inhibitory. The IP was also inhibited by EDTA and F − but not by iodoacetamide.
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