Aliifodinibius salicampi sp. nov., a moderately halophilic bacterium isolated from a grey saltern.

Abstract

Three strains of a Gram-stain-negative, moderately halophilic bacterium, designated strain KHM44T, KHM29 and HHM4, were isolated from the sediment of a grey saltern located on Sinui island at Shinan, Korea. The isolates were aerobic, non-motile rods and grew at 15-50 °C (optimum, 37 °C), at pH 6.0-9.0 (optimum, pH 8.0) and at salinities of 3-25 % (w/v) NaCl (optimum, 10 % NaCl). The predominant isoprenoid quinone was menaquinone-7 (MK-7), and the major fatty acids were iso-C15 : 0, C16 : 1ω7c and/or iso-C15 : 0 2-OH, iso-C17 : 1ω9c and anteiso-C15 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and glycolipid. The DNA G+C contents were 48.5-48.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains KHM44T, KHM29 and HHM4 belonged to the genus Aliifodinibius in the family Balneolaceae, with sequence similarities of 95.1-97.2 % to members of this genus. Levels of DNA-DNA relatedness between strain KHM44T and the type strains of the other species of the genus Aliifodinibius ranged from 35.4 to 48.0 %. On the basis of polyphasic analysis from this study, strains KHM44T, KHM29 and HHM4 are considered to represent a novel species of the genus Aliifodinibius, for which the name Aliifodinibius salicampi sp. nov. is proposed. The type strain is KHM44T (=KACC 19060T=NBRC 112531T).