Abstract
Neural tissue regeneration is a significant challenge, because severe nerve injury is quite difficult to regenerate spontaneously. Although, many studies have been devoted to promote nerve regeneration, there are still many technical challenges to achieve satisfactory results. In this study, we designed biomimetic matrices composed of aligned laminin core-polydioxanone/collagen shell (Lam-PDO/Col) fibers, which can provide both topographical and biochemical cues for promoting neuritogenesis. The aligned Lam-PDO/Col core-shell fiber matrices were fabricated by magnetic field-assisted electrospinning with the coaxial system, and their potential as biofunctional scaffolds for promoting neuritogenesis was explored. It was demonstrated that the aligned Lam-PDO/Col core-shell fibers were successfully fabricated, and the laminin in the core of fibers was steadily and continuously released from fibers. In addition, the cellular behaviors of hippocampal neuronal cells on the matrices were significantly enhanced. Moreover, the aligned Lam-PDO/Col fiber matrices effectively improved and guided neurite outgrowth as well as the neurogenic differentiation by providing both topographical and biochemical cues through aligned fiber structure and sustained release of laminin. Collectively, it is suggested that the aligned Lam-PDO/Col core-shell fiber matrices are one of the most promising approaches for promoting neuritogenesis and neural tissue regeneration.
Highlights
Nerve injury is often caused by resections of tumors, traffic accidents or iatrogenic side effects of the surgery[1]
fast Fourier transform (FFT) data images of the aligned fiber matrices result in an output image where pixels are distributed in an elliptical shape since the pixel intensities are distributed to a specific orientation
The main contributions of this study can be summarized as follows: (1) We successfully fabricated the aligned Lam-PDO/Col core-shell fibers, which can provide both topographical and biochemical cues for promoting neuritogenesis, by magnetic field-assisted electrospinning with the coaxial system
Summary
Nerve injury is often caused by resections of tumors, traffic accidents or iatrogenic side effects of the surgery[1]. Much research have focused on the development of artificial scaffolds to enhance the neuronal cell behaviors by a tissue engineering approach Several techniques, such as gas forming, salt leaching and electrospinning, have been employed for fabricating artificial scaffolds that can mimic the natural extracellular matrix (ECM) strucuture[4,5,6]. We designed fibrous matrices composed of aligned laminin core-polydioxanone/collagen shell (Lam-PDO/Col) fibers, which can provide both topographical and biochemical cues for promoting neuritogenesis, and investigated their effects on neuronal cell regeneration. We fabricated aligned Lam-PDO/ Col core-shell fiber matrices by magnetic field-assisted electrospinning with the coaxial system. The cellular behaviors of HT-22 mouse hippocampal neuronal cells on the matrices were evaluated to examine their potentials as tissue engineering scaffolds for neuritogenesis and neural tissue regeneration
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