Abstract
In vitro production has been employed in bovine embryos and quantification of lipids is fundamental to understand the metabolism of these embryos. This paper presents a unsupervised segmentation method for histological images of bovine embryos. In this method, the anisotropic filter was used in the differents RGB components. After pre-processing step, the thresholding technique based on maximum entropy was applied to separate lipid droplets in the histological slides in different stages: early cleavage, morula and blastocyst. In the postprocessing step, false positives are removed using the connected components technique that identify regions with excess of dye near pellucid zone. The proposed segmentation method was applied in 30 histological images of bovine embryos. Experiments were performed with the images and statistical measures of sensitivity, specificity and accuracy were calculated based on reference images (gold standard). The value of accuracy of the proposed method was 96% with standard deviation of 3%.
Highlights
In vitro production (IVP) has been employed to improved features genetics of bovine embryos [1]
This paper presents a unsupervised segmentation method for histological images of bovine embryos
The method’s ability to identify negative results is important: Early cleavage with 87%, Morula with 78% and Blastocyst with 88%, where these values were obtained with R colour component
Summary
In vitro production (IVP) has been employed to improved features genetics of bovine embryos [1]. The increase in the levels of cytoplasmic lipid droplets in the IVP of bovine embryos are relationship reduction of embryos after cryopreservation [2]. Quantify the lipids is fundamental to understand the metabolism of these embryos. Some methods are applied to evaluate and measure the features of lipids in the investigation of IVP of bovine embryos. The Sudan black B staining is a method that consists of a lipophilic dye, which reacts with lipids. Regions with lipids are represented by darker colours compared to the rest of the embryo without lipid droplets. After Sudan black B staining of bovine embryos, a microscope equipped with digital camera is used to acquire images [3]
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