Algoriphagus marisflavi sp. nov., isolated from water of an estuary environment.

Abstract

A Gram-stain-negative, non-motile, aerobic and rod-shaped or ovoid bacterial strain, designated KEM-106T, was isolated from water of an estuary environment on the Yellow Sea, South Korea, and subjected to a polyphasic taxonomic study. Strain KEM-106T grew optimally at 30 °C and pH 7.0-8.0 and in the presence of 1.0-2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain KEM-106T belonged to the genus Algoriphagus, clustering with the type strains of Algoriphagus litorisediminis and Algoriphagus aquaemixtae. Strain KEM-106T exhibited 16S rRNA gene sequence similarities of 96.5, 96.2 and 96.0 % to the type strains of Algoriphagus boritolerans, A. litorisediminis and A. aquaemixtae, respectively, and of 92.5-95.8 % to the type strains of the other Algoriphagus species. Strain KEM-106T contained MK-7 as the predominant menaquinone and iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the major fatty acids. The major polar lipids were phosphatidylethanolamine and phosphatidylcholine. The DNA G+C content of strain KEM-106T was 42.7 mol%. Differential phenotypic properties, together with its phylogenetic distinctiveness, revealed that strain KEM-106T is separated from recognized species of the genus Algoriphagus. On the basis of the data presented, strain KEM-106T is considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus marisflavi sp. nov. is proposed. The type strain is KEM-106T (=KCTC 52979T=NBRC 112904T).