Abstract
Abstract: 1) The research was purposed production of oligosaccharide from alginate hydrolysis the main composition in cell walls of sea weed. We was isolated 252 strains from sea water and mud flat, the highest alginate lyase activity was selected, and identified as Sanguibacter keddieii NC9 by 16S rDNA sequence analysis. In this study was select the sodium alginate concentration, pH, temperature for the production of alginate lyase activity. Alginate lyase activity was confirmed from plate assay with 10% cetylpyridinium chloride. The optimum culture conditions for the production of alginate lyase were sodium alginate 10 g/L, peptone 5 g/L, 40 ℃, pH 9 and 36 hours incubation time. Sanguibacter keddieii NC9, its alginate lyase would be useful for the production of bioenergy and biofunctional oligosaccharides from sea weed. Keywords: Alginate lyase, sea water, enzymatic activity, brown algae 1. 서론 해조류는 전 세계적으로 약 200 여종이 식용 및 산업적 활용을 위해 이용되고 있으며 연간생산량은 약 1,500만 톤에 달한다. FAO에 따르면 전 세계 해조류 생산량은 중국이 약
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