Alginate hydrogel with enhanced curcumin release through HPβCD assisted host-guest interaction

Abstract

The underprivileged pharmacodynamic action of curcumin, which arose from its low water solubility and rapid metabolism, restricts its therapeutic performance. In this study, (2-Hydroxy isopropyl)-β-cyclodextrin (HPβCD) as a macrocycle host molecule was employed to enhance the availability and control release of curcumin by forming a host-guest inclusion complex within an in-situ forming alginate hydrogel. The formation of the inclusion complexes of curcumin with a single host molecule was characterized by FTIR, XRD, TGA, SEM, and DLS analyses. The inclusion complex of curcumin and HPβCD (HPβCD-Cur) showed a high encapsulation efficiency of 88.2 %. According to DLS results, aqueous dispersion of HPβCD-Cur exhibited a unimodal histogram after 2 and 7 days with average particles size of 207.5 and 230.6 nm, respectively. This observation could be because of the formation of an inclusion complex that effectively distributed in solution and prevented curcumin agglomeration. The prepared alginate hydrogel containing HPβCD-Cur demonstrated >87 % reduction in colonies of methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa, which significantly is higher than that for Alg/Cur (<69 %). The Alg/HPβCD-Cur hydrogel exhibited a high water uptake of 470 % after 2 h, and a curcumin cumulative release of 80 % over 72 h, with proper cytocompatibility. Consequently, it was shown that the HPβCD carrier could act as an apt host molecule that can properly encapsulate curcumin and enhance its release from the Alg/HPβCD-Cur hydrogel.