Abstract
In the present study, Lactobacillus acidophilus LA-5 was microencapsulated in sodium alginate, followed by fish gelatin coating (0.5, 1.5, and 3%). The survival of L. acidophilus in bread before and after encapsulation in alginate/fish gelatin during the baking and 7-day storage was investigated. Moreover, the effect of alginate/fish gelatin-encapsulated L. acidophilus on the technological properties of bread (hardness, staling rate, water content, oven spring, specific volume, and internal texture structure) was evaluated. Compared with control (free bacteria), encapsulated L. acidophilus in alginate/fish gelatin showed an increase in the viability of bread until 2.49 and 3.07 log CFU/g during baking and storage, respectively. Good viability of (106 CFU/g) for probiotic in encapsulated L. acidophilus in alginate/fish gelatin (1.5 and 3%, respectively) after 4-day storage was achieved. Fish gelatin as a second-layer carrier of the bacteria had a positive effect on improving the technical quality of bread. Furthermore, the staling rate of bread containing encapsulated L. acidophilus alginate/fish gelatin 0.5, 1.5, and 3% decreased by 19.5, 25.8, and 31.7%, respectively. Overall, the findings suggested encapsulation of L. acidophilus in alginate/fish gelatin capsule had great potential to improve probiotic bacteria’s survival during baking and storage and to serve as an effective bread enhancer.
Highlights
Bread is an innovative field in the probiotic food sector and has attracted rising interest in research
Probiotics are live bacteria and yeasts promoted as having health benefits to the host when consumed adequately
The Lactobacillus acidophilus (LA-5) probiotic strain was provided by Christian Hansen (Horsholm, Denmark)
Summary
Bread is an innovative field in the probiotic food sector and has attracted rising interest in research. Due to the high temperatures during the bread baking process, probiotics in bread are challenging [1]. Probiotics are live bacteria and yeasts promoted as having health benefits to the host when consumed adequately. These microbes must survive the processing and storage of food, as the presence of large numbers of unstressed and viable microbial cells at the time of using them is responsible for the health benefits. A promising strategy to enhance probiotic bacteria’s survival is to encapsulate probiotic cells in a protective capsule. Zhang et al [5] encapsulated
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
