Abstract
Protocols for isolation, characterization, and transplantation of hematopoietic stem cells (HSCs) have been well established. However, difficulty in finding human leucocyte antigens (HLA)-matched donors and scarcity of HSCs are still the major obstacles of allogeneic transplantation. In this study, we developed a double-layered microcapsule to deliver paracrine factors from non-matched or low-matched HSCs to other cells. The umbilical cord blood-derived hematopoietic progenitor cells, identified as CD34+ cells, were entrapped in alginate polymer and further protected by chitosan coating. The microcapsules showed no toxicity for surrounding CD34+ cells. When CD34+ cells-loaded microcapsules were co-cultured with bare CD34+ cells that have been collected from unrelated donors, the microcapsules affected surrounding cells and increased the percentage of CD34+ cell population. This study is the first to report the potency of alginate-chitosan microcapsules containing non-HLA-matched cells for improving proliferation and progenitor maintenance of CD34+ cells.
Highlights
Licensee MDPI, Basel, Switzerland.Hematopoietic stem cells (HSCs) are multipotent cells capable of generating all blood components, including erythrocytes, leucocytes, and thrombocytes [1]
The core is alginate-entrapped cells, and the outer layer consists of chitosan cross-linked with glutaraldehyde (Figure 1B,D)
Co-cultures produced higher percentages of CD34+ cells than of monoculture (Figure S3B). These results suggested that co-culture with encapsulated cells, isolated from an unrelated donor, have beneficial effects for CD34+ cell expansion and progenitor maintenance
Summary
Hematopoietic stem cells (HSCs) are multipotent cells capable of generating all blood components, including erythrocytes, leucocytes, and thrombocytes [1]. HSCs represent only a fraction of the cell population in umbilical cord blood, bone marrow, or peripheral blood. To isolate the HSCs from heterogenous populations, specific surface markers such as HLA-DR, CD38, CD117 (c-kit), CD45, CD133, and/or CD34 have been used [2]. One of the prominent surface markers that is widely used to isolate hematopoietic progenitor. CD34+ cells that have enhanced progenitor activity represent a small proportion of the population [4]. Previous studies have reported that transplantation of CD34+ cells successfully established multi-lineage hematopoietic engraftment [5] and improved neurobehavior of animals with stroke [6,7], brain injury [8], and cerebral palsy [9]
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