Alginate based 3D micro-scaffolds mimicking tumor architecture as in vitro cell culture platform

Abstract

A micron scale alginate based 3D platform embedded with a carbon dot pH sensor, that enables continuous growth monitoring of encapsulated cells in real time is reported. The alginate based 3D micro-scaffold closely mimics a tumor microenvironment by providing a spatial demarcation and making it possible to encapsulate different cells in close proximity. The micro-scaffold contains carbon dot based nanosensors that enable real time monitoring of pH change in the tumor microenvironment avoiding the need for end-point assays for studying cellular growth. The micro-scaffolds have heterogeneous architecture and a hypoxic core region can be observed in as less as 96 h of culture. In this completely synthetic platform, there also exist the flexibility of artificially modifying the porosity of the micro-scaffold as per the requirement of the studies where a denser ECM mimic is required. The micro-scaffolds were conducive for cell growth as suggested by the enhanced functional profile of hepatocellular carcinoma cells and positively influence the genetic expression of the cell specific markers. Additionally, similar to a 3D tumor, non-homogeneous diffusion of molecules is also observed making this an ideal platform for cancer modelling and drug screening.