Algal filtrate: a low cost substitute to synthetic growth regulators for direct organogenesis of embryo culture in Jatropha curcas (Ratanjyot)

Abstract

Jatropha curcas, the energy plant has attained great attention in recent years because of its biodiesel production potential and medicinal value. This makes it imperative to search for techniques for its rapid propagation. Our research communication has shown for the first time direct organogenesis without callus formation from embryo culture of Jatropha. All previous reports embody callusing before further propagation and use of whole seeds. We also report the very economical protocol for J. curcas using cyanobacterial culture filtrate (Aulosira fertilissima) in place of chemical hormones giving this paper a cutting edge to in vitro propagation of J. curcas. The result showed that the number of days taken for shoots and root induction was quicker by adding the cyanobacterial filtrate and shoot and root length was comparatively higher than the other treatment with synthetic plant growth regulator. The same trend was found for chlorophyll a and b. No such report previously has ever focused on the use of cyanobacterial filtrate on in vitro germination of J. curcas embryo to regenerate plants at a faster rate. Ex vitro rooting is a new approach, which will reduce the time for regeneration still further, is an area that is being presently tried out.