Abstract

Algal biomass that is represented mainly by commercially grown algal strains has recently found many potential applications in various fields of interest. Its utilization has been found advantageous in the fields of bioremediation, biofuel production and the food industry. This paper reviews recent developments in the analysis of algal biomass with the main focus on the Laser-Induced Breakdown Spectroscopy, Raman spectroscopy, and partly Laser-Ablation Inductively Coupled Plasma techniques. The advantages of the selected laser-based analytical techniques are revealed and their fields of use are discussed in detail.

Highlights

  • Expanding economies are still technologically dependent on crude oil, while the decreasing amount of oil deposits moves up prices of crude oil and related gasoline [1]

  • In this review the literature was surveyed for recent developments and results in research utilizing selected laser-based techniques for analysis of the algal biomass or calcified coralline algae

  • On the contrary, when other approaches are used for analysis where the part of a sample is dissolved/pelleted and analyzed using for instance methods based on atomic absorption spectroscopy (AAS) or MALDI information about spatial location and time evolution is hard to obtain or even completely lost

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Summary

Introduction

Expanding economies are still technologically dependent on crude oil, while the decreasing amount of oil deposits moves up prices of crude oil and related gasoline [1]. Pořízka et al [60] used laser-based techniques (LIBS and Raman spectroscopy) in tandem for obtaining both elemental and molecular information of the algal strain Trachydiscus minutus. The classification of the algal strains based on their spectra, i.e., spectrochemical fingerprint in the sense of elemental or molecular composition, can be provided by employing standard chemometric algorithms, such as principal component analysis (PCA) [61] and partial least squares (PLS) algorithms [61,62] Chemometrics and their applications are further discussed in the text. In this review the literature was surveyed for recent developments and results in research utilizing selected laser-based techniques (mainly LIBS and Raman spectroscopy, partly LA-ICP-MS) for analysis of the algal biomass or calcified coralline algae. On the contrary, when other approaches are used for analysis where the part of a sample is dissolved/pelleted and analyzed using for instance methods based on atomic absorption spectroscopy (AAS) or MALDI information about spatial location and time evolution is hard to obtain or even completely lost

Laser-Induced Breakdown Spectroscopy
Laser-Induced Breakdown Spectroscopy of Liquid Samples
Laser-Induced Breakdown Spectroscopy for Molecular Analysis
Laser Ablation Inductively Coupled Plasma Based Techniques
Raman Spectroscopy
Chemometrics for the Recognition of Algal Strains
Discrimination of Four Algal Strains by LIBS
Findings
Conclusions and Future Prospects
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