Abstract
From two strains of alfalfa mosaic virus (strain 425 and yellow spot mosaic virus) which differ in component composition, serotype, symptoms induced in tobacco and bean, and sensitivity to cycloheximide, the three nucleoprotein components required for infectivity were purified, all possible combinations were made, and the progeny consisting of stable hybrids were characterized. This study resulted in the localization of the markers on the different component RNAs. Top component b RNA determines the serotype, the kind of symptoms on tobacco, the relative proportions of the components, and the sensitivity to cycloheximide. Middle component RNA determines the kind of symptoms on bean. No markers have been found on the bottom component RNA.
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