Abstract
Extracts of alfalfa leaf meal may increase or decrease the mycelial weight of Neurospora sitophila in the standard Difco pyridoxine assay. The effect obtained was found to be dependent upon the solvent employed or the subsequent treatment of the extract. Water extracts were inactive or inhibitory. Extracts with 0.1 N or stronger hydrochloric acid were active. Increasing the temperature or time of extraction favored activation only slightly. The inactive water extracts could be activated by the addition of hydrochloric acid or by the extraction of inhibitory material with diethyl ether or dimethylformamide.
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