Abstract
Primary cultures of immunodissected cells from rabbit kidney connecting tubule and cortical collecting duct were used to study aldosterone's action on transcellular Na + flux. Incubation with 10 −7 M aldosterone stimulated transcellular Na + transport which was detected as an increase in benzamil-sensitive short-circuit current. The stimulatory response was consistently noted after 2 h of incubation and stabilized after 6 h. 2D-PAGE was used to identify proteins which were induced concurrently with the increase in transcellular Na + flux after an aldosterone incubation of 15 h. Three aldosterone-induced proteins (AIPs; M r = 100, 70–77 and 46–50 kDa) were found in the membrane and microsomal fractions. Two of these appeared to have more than one isoform. A single heterogeneous AIP (M r = 77 kDa) was detected in the soluble fraction.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Biomembranes
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