Abstract
Diabetes complications, including peripheral neuropathy, cataracts, impaired wound healing, vascular damage, arterial wall stiffening and retinopathy diseases, are among the most predominant health problems facing the world’s population today. The 22 Peruvian plant extracts were screened for their potential inhibitory activity against rat lens aldose reductase (RLAR) and DPPH radical scavenging. Among them, we have found that Tanacetum parthenium L. (TP) has the RLAR, AGEs and DPPH radical scavenging activities. We used for screening of active components in TP against RLAR and DPPH for the first time by ultrafiltration (UF) and DPPH. Compounds in TP were isolated by Sephadex column chromatography and their structures were established by MS and NMR spectroscopic analyses. Among the isolated compounds, ferulic acid, apigenin, luteolin-7-O-glucoside, luteolin, chrysosplenol, and kaempferol showed potent inhibition with IC50 values of 1.11–3.20 and 6.44–16.23 μM for RLAR and DPPH radical scavenging. Furthermore, these compounds suppressed sorbitol accumulation in rat lenses and ferulic acid, luteolin-7-O-glucoside, and luteolin have AGEs inhibitory activities with IC50 values of 3.43–6.73 μM. In summary, our study provides interesting plants for further study with respect to the treatment and prevention of diabetic complication of Peruvian plant and can provide the scientific base of the traditional uses.
Highlights
Aldose reductase (AR: EC 1.1.1.21) is the rate-limiting enzyme of the polyol pathway
AR-catalyzed formation of sorbitol has been observed in a number of tissues; in diabetes mellitus, increased sorbitol generated through the polyol pathway does not readily diffuse across cell membranes, and intracellular accumulation of sorbitol has been implicated in chronic complications of diabetes, such as cataracts, neuropathy, and retinopathy [3]
The results demonstrate that compound 4 possesses the highest reduction of peak area (PAR; 29.36%) followed by 1 (23.53%), 3 (16.85%), 6 (13.97%), 2 (11.28%), 5 (7.19%), and 7 (0%), which might be caused by their structures and concentrations (Table 3)
Summary
Aldose reductase (AR: EC 1.1.1.21) is the rate-limiting enzyme of the polyol pathway. AR-catalyzed formation of sorbitol has been observed in a number of tissues; in diabetes mellitus, increased sorbitol generated through the polyol pathway does not readily diffuse across cell membranes, and intracellular accumulation of sorbitol has been implicated in chronic complications of diabetes, such as cataracts, neuropathy, and retinopathy [3]. The nonenzymatic reaction leads to chemical modifications of tissue proteins, called advanced glycation end products (AGEs), resulting in functional disturbances of the proteins [4]. Both diabetes and aging are associated with accumulation of AGEs in tissues, increased oxidative stress, and decline in antioxidant status. The complex, fluorescent AGE molecules formed during the Maillard reaction can lead to protein cross-linking and contribute to the development and progression of diabetic complications, such as peripheral neuropathy, cataracts, impaired wound healing, vascular damage, arterial wall stiffening, and decreased myocardial compliance [6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
