Abstract
Aldo-keto reductase family 1C (AKR1C) members transform steroids via their 3-, 17-, and 20-ketosteroid reductase activities. The biochemical study of these enzymes can help to inform their roles in hormone-dependent diseases and develop therapeutic inhibitors. This work describes a protocol to purify AKR1C1-4 members from a bacterial expression system using two chromatography steps. It also describes the basis of discontinuous assays to measure steroid conversion.
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