Alcohol oxidase ofAspergillus flavipesgrown on hexadecanol

Abstract

The activity of alcohol oxidase in Aspergillus flavipes was induced by growth on hexadecanol, though highest activities were obtained using a mixture of hexadecanol and olive oil. The enzyme showed a wide range of substrate specificity towards aliphatic primary alcohols from C8 to C18. The preferred substrate was decanol. The enzyme had an optimum pH of 9.5. It also used cis-unsaturated alcohols better than the trans-isomers. ω-Hydroxy fatty acids and α,ω-diols were not attacked.