Abstract
Extracellular vesicles are nanosized vesicles that are under intense investigation for their role in intercellular communication. Extracellular vesicles have begun to be examined for their role in disease protection and their role as disease biomarkers and/or vaccine agents. The goal of this study was to investigate the effects of alcohol exposure on the biogenesis and composition of extracellular vesicles derived from the cervical cancer line, HeLa. The HeLa cells were cultured in exosome-free media and were either mock-treated (control) or treated with 50 mM or 100 mM of alcohol for 24 h and 48 h. Our results demonstrated that alcohol significantly impacts HeLa cell viability and exosome biogenesis/composition. Importantly, our studies demonstrate the critical role of alcohol on HeLa cells, as well as HeLa-derived extracellular vesicle biogenesis and composition. Specifically, these results indicate that alcohol alters extracellular vesicles’ packaging of heat shock proteins and apoptotic proteins. Extracellular vesicles serve as communicators for HeLa cells, as well as biomarkers for the initiation and progression of disease.
Highlights
The World Health Organization (WHO)-International Agency for Research on Cancer (IARC)(2009) has classified alcoholic beverages as a Group 1 Carcinogen
Here, we evaluated the affect of alcohol administration over time on the cervical cancer cell line, Human cervical epithelial (HeLa)
HeLa cells not treated or treated with EtOH at 50 mM or 100 mM in the exosome-free medium for 24 h and 48 h were evaluated for cell viability
Summary
The World Health Organization (WHO)-International Agency for Research on Cancer (IARC)(2009) has classified alcoholic beverages as a Group 1 Carcinogen (carcinogenic to humans). Madsen et al found a higher risk of VV-SCC in women that had high alcohol consumption [1]. With the high mortality rate of cervical cancers, more information is needed regarding detection and treatment of the diseased. In this regard, several investigators have begun to examine extracellular vesicles (EVs) and their role in disease protection [2], as disease biomarkers [3,4,5] and/or as a cargo/vaccine agents for cervical cancer [6,7,8,9,10,11,12,13,14,15,16,17,18].
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