Alcohol exacerbated nitroxidative stress in brain of diabetic rats: An ameliorative role of green tea

Abstract

Alcohol abuse and diabetes exist in many populations as comorbities. Alcoholism and diabetes can induce wide spectrum of effects on central nervous system. This study focussed on, the impact of nitroxidative stress in alcohol treated diabetic rats and to evaluate the possible protective effect of aqueous extract of green tea (GTE). Male albino Wistar rats aged of 8 weeks were made diabetic with steptozotocin (STZ) and treated with at 5 g/kg b.w/day (20% v/v) alcohol for sixty days. Alongside enhanced reactive oxygen species (ROS), reactive nitrogen species (RNS) levels and neuronal nitric oxide synthase (nNOS) expression were found to be increased and activities of antioxidant enzymes were decreased in the brain of alcohol treated diabetic rats. When the alcohol was administered to the diabetic rats, it is difficult to know whether the observed changes are independent or overlapping effects. Increased expression of mRNAs of Bcl, Bax, and TNF-α, IL-6, Caspase-3, and COX2 genes suggest that they may have functional significance about alcohol intoxication. This study provided information that, green tea catechin, (-)-epigallocatechin-3-O-gallate (EGCG) has a therapeutic effect and thus ameliorate the effect of alcohol in diabetic condition.