Abstract
Isozyme pattern were studied in six teleost species of Catla (Catla catla), Rohu (Labeo rohita), Mrigal (Cirrhinus mrigala), Common carp (Cyprinus carpio), Grass carp (Ctenopharyngodon idella) and Silver carp (Hypophthalmichthyes molitrix) from gill, muscle, heart and kidney tissues. Alcohol dehydrogenase (ADH) were separated in native-PAGE of 7.5% gradient. ADH-1 and ADH-2 loci were observed and comparison of Rm value of the polypeptide show different diversified enzyme activities which were sync with their feeding metabolic, habitat and environmental factors. The overall observation was found to be tissue specific.
Highlights
Alcohol dehydrogenase (ADH; alcohol: NAD+ oxidoreductase, EC 1.1.1.1) is the enzyme that catalyzes the interconversion of alcohols with the corresponding aldehydes and ketones and are widely distributed in micro organisms, plants and animals [25]
Population study can aid in the management of fish populations [28] and a large amount of genetic differentiation can occur within a small geographic area [29]
Since there is no competition for food and fear for predators, a farmed fish population experiences different kinds of selection regimes unprecedented in natural waters
Summary
Alcohol dehydrogenase (ADH; alcohol: NAD+ oxidoreductase, EC 1.1.1.1) is the enzyme that catalyzes the interconversion of alcohols with the corresponding aldehydes and ketones and are widely distributed in micro organisms, plants and animals [25]. Molecular genetic markers have been applied to three fisheries areas in particular-stock structure analysis, aquaculture and taxonomy/systematic [27] with varying degree of success [3] All these techniques have their advantages and disadvantages in analysis of genetic variability in fishes [26, 8]. The isozyme electrophoretic technique is ideally suited to population studies as it is fairly rapid procedure to perform on a large scale, and a large number on unlinked loci that are dispersed throughout the genome can be screened simultaneously [16] It is relatively inexpensive and requires little in the way of specialized equipment. Catla (Catla catla), Rohu (Labeo rohita), Mrigal (Cirrhinus mrigala), Common carp (Cyprinus carpio), Grass carp (Ctenopharyngodon idella) and Silver carp (Hypophthalmichthyes molitrix) has been carried out
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