Abstract
Sarcopenia is an aging associated disorder involving skeletal muscle atrophy and a reduction in muscle strength, and there are no pharmaceutical interventions available thus far. Moreover, conditions such as hyperglycaemia are known to further intensify muscle degradation. Therefore, novel strategies to attenuate skeletal muscle loss are essential to enhance muscle function and thereby improve the quality of life in diabetic individuals. In this study, we have investigated the efficiency of a potato peptide hydrolysate PPH902 for its cytoprotective effects in skeletal muscle cells. PPH902 treatment in C2C12 cells showed the dose-dependent activation of the Akt/mTOR signalling pathway that is involved in skeletal myogenesis. According to Western blotting analysis, PPH902 induced the phosphorylation of Akt, mTOR proteins and induced the myogenic differentiation of C2C12 myoblasts in a differentiation medium. The phosphorylation myogenic transcription factor Foxo3A was also found to be increased in the cells treated with PPH902. In addition, treatment with PPH902 ameliorated the high glucose induced reduction in cell viability in a dose-dependent manner. Moreover, the number of myotubes in a differentiation medium reduced upon high glucose challenge, but treatment with PPH902 increased the number of differentiated myotubes. Further, the phosphorylations of AMPK and mitochondrial-related transcription factors such as PGC-1α were suppressed upon high glucose challenge but PPH902 treatment restored the protein levels. We demonstrate, for the first time, that a specific potato peptide has a therapeutic effect against sarcopenia. In addition, PPH902 improved the myogenic differentiation and their mitochondrial biogenesis and further improved myogenic protein and inhibited muscle protein degradation in C2C12 cells challenged under a high glucose condition.
Highlights
IntroductionAging is inevitably associated with a progressive decline in bodily function and composition with a decline in muscle strength and mass, a phenomenon known as sarcopenia
In order to determine thethe effect of PPH902 on on thethe viability of C2C12 cells, MTT
Was performed, andand the the results showed no toxic effect of PPH902 on C2C12 cells even up toup 10 to performed, results showed no toxic effect of PPH902 on C2C12 cells even μg/mL
Summary
Aging is inevitably associated with a progressive decline in bodily function and composition with a decline in muscle strength and mass, a phenomenon known as sarcopenia. The consequence of this effect includes a reduction in aerobic capacity, causing reduced mobility and quality of life in elderly subjects. The annual cost, in the United States, of the health effects of sarcopenia accounts to USD 18.5 billion and the scenario is likely to be global as the aging population grows in most developed countries [1]. The likely to be global as the aging population grows in most developed countries [1]. The present treatment strategy recommends protein nutritional uptake and physical to maintain muscle function [3,4].nutritional uptake and physical exercise to presentexercise treatment strategy recommends protein maintain muscle function [3,4]
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