Abstract
A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use.•Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample•The limit of detection of our developed platform is 0.05 µg.mL−1•The detection range of our aptasensor is 0.1–600 µg.mL−1
Highlights
Method ArticleAlbuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
The limit of detection of our developed platform is 0.05 μg.mL−1 The detection range of our aptasensor is 0.1–600 μg.mL−1
It can be used for the detection of HSA in urine without sample dilution because the albuminuria concentration in normal urine is usually lower than 30 μg.mL−1 [3]
Summary
Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor. Wireeya Chawjiraphan a, Chayachon Apiwat a,c, Khoonsake Segkhoonthod a, Kiatnida Treerattrakoon a,f, Preedee Pinpradup a, Nuankanya Sathirapongsasuti b, Prapasiri Pongprayoon c,d, Patraporn Luksirikul c, Patcharee Isarankura-Na-Ayudhya e, Deanpen Japrung a,∗. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. Method name: Albuminuria detection by graphene oxide-mediated fluorescence quenching aptasensor. Biochemistry, Genetics and Molecular Biology Nanotechnology for medical application Albuminuria detection by graphene oxide-mediated fluorescence quenching aptasensor. Sensitive detection of albuminuria by graphene oxide-mediated fluorescence quenching aptasensor.
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