Albumin from Erythrina edulis (Pajuro) as a Promising Source of Multifunctional Peptides.

Cleni Palma-Albino,Nathaly Rodríguez-Arana,Amparo Iris Zavaleta,Karim Jiménez-Aliaga,Estela Solano,Blanca Hernández-Ledesma,Arturo Intiquilla,Eduardo Flores,Víctor Izaguirre

doi:10.3390/antiox10111722

Abstract

Multifunctional peptides, capable of acting on different body systems through multiple mechanisms of action, offer many advantages over monofunctional peptides, including lower adverse side effects and costs. Erythrina edulis (pajuro) is a legume with a large number of high-quality proteins, of which their potential as a source of antioxidant peptides has been recently reported. In this study, the behavior of these proteins under a sequential enzymatic hydrolysis with digestive and microbial enzymes was investigated by evaluating the multi-functionality of the hydrolyzates. The albumin hydrolyzates obtained after the action of pepsin, pancreatin, and Alcalase showed antioxidant, angiotensin-converting enzyme (ACE), α-amylase, α-glucosidase, and dipeptidyl peptidase (DPP)-IV inhibitory activities. The radical scavenging properties of the hydrolyzate could be responsible for the potent protective effects observed in FeSO4-induced neuroblastoma cells. The findings support the role of pajuro protein as an ingredient of functional foods or nutraceuticals for health promotion and the prevention of oxidative stress, hypertension, and metabolic alteration-associated chronic diseases.

Highlights

Proteins are vital macronutrients involved in body maintenance and growth; the search for high-quality proteins and their inclusion into the daily diet has become a prevalent research area
The values of ash, fat, and fiber content determined in the E. edulis seed flour were 39.5 g/kg, 13.1 g/kg, and 58.9 g/kg, respectively
We found that the level of Lactate Dehydrogenase (LDH) was significantly increased in FeSO4 -induced cells (184.65% compared to non-induced cells), while treatment with the hydrolyzate the LDH levels in the supernatant were all significantly reduced in SH-SY5Y cells, reaching values lower than that of control cells (42.16% at dose of 1.0 mg/mL) (Figure 4D)

Summary

Introduction

Proteins are vital macronutrients involved in body maintenance and growth; the search for high-quality proteins and their inclusion into the daily diet has become a prevalent research area. Traditional and under-utilized plants are becoming popular as a source of proteins that provide important nutritional, technological, and functional properties [1]. In addition to these attributes, proteins provide multiple health benefits through their impact on specific biochemical pathways. Numerous bioactive peptides have been identified from plant foods protein hydrolyzates Their diversified structures explain the wide range of activities demonstrated for these peptides, such as antioxidant, anti-inflammatory, anti-hypertensive, anti-microbial, anti-diabetic, and chemo-preventive activities, among others [3,4,5]. These reported effects demonstrate the potential of bioactive peptides to be used as ingredients of functional

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