Abstract
Superoxide anion radical (O 2 •− ) is a toxic reactive oxygen species (ROS). Schiff-base metal complexes have been widely studied as synthetic antioxidants to scavenge ROS. However, it is toxic and shows poor water solubility. In this work, a kind of novel water-soluble biopolymer/metal complex conjugate (HO-SAM@BSA) was prepared by binding the 4-hydroxy-salicylaldehyde amino acid Schiff-base metal complexes (HO-SAM, M = Cu, Zn, Co) with water-soluble biopolymer bovine serum albumin (BSA). The conjugates were characterized using IR, UV–Vis, circular dichroism spectra (CD), and polyacrylamide gel electrophoresis (PAGE). The results show that the structure of BSA is maintained when the binding rate (nHO-SAM: nBSA) of amino acid Schiff-base metal complexes is 10. In addition, the O 2 •− scavenging activities of resultant conjugates were determined via nitrobule tetrazolium assay method. After combining HO-SAM into BSA, the poor water-solubility of HO-SAM is improved, and the O 2 •− scavenging activity of BSA increases dramatically. The conjugation HO-SCCu@BSA displays excellent O 2 •− scavenging activity. When the EC50 value was 0.10 μmol/L, the analog quantity reached 41 % of natural SOD. Therefore, it can act as a bifunctional mimic of enzyme, which has a great application prospect in antioxidant drug.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Inorganic and Organometallic Polymers and Materials
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
