Abstract
Abstract Inhibition of growth by d-cycloserine of a clinical isolate of Escherichia coli in a synthetic alanine-free broth medium was blocked by the addition of d-alanine in equimolar or greater amounts. Heating a synthetic broth medium containing the l-isomer of alanine to 115 ° C. resulted in subversion of the antibiotic effect of d-cycloserine on E. coli. Comparable heating with d-alanine resulted in loss of capacity for antagonism toward d-cycloserine-mediated antibiosis. Chemical analysis for d-alanine showed that synthetic broth medium containing l-alanine contained d-alanine following autoclave sterilization. Three commercial broth media, in common use for conventional antibacterial agent susceptibility testing, contained d-alanine before and after autoclave sterilization.
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