Abstract
Mast cells are dispersed in many tissues, especially in digestive and respiratory tract mucosal membranes. Tryptase, regarded as marker of mast cells activity is the most important proteinase released from these cells during degranulation. It influences cells and tissues activating inflammatory process. The aim of the study was to assess the activity of tryptase in colon mucosa in children with inflammatory bowel diseases. Materials and methodsThe investigated group, which consisted of 30 patients with IBD was divided into 3 subgroups according to type of IBD: ulcerative colitis (UC), Crohn's disease (CD) and non-specific colitis (NSC). The diagnosis was based on colonoscopy. The control group comprised 33 children with gastrointestinal bleeding, who presented no abnormalities on colonoscopy. The concentration of tryptase in the studied samples was evaluated by fluoroimmunoenzymatic method (FEIA) with the use of UniCAP (Pharmacia &Upjohn Diagnostics AB) after homogenization of the sample. The results were analysed statistically with programme Statistica 9.1. ResultsThe mean concentration of tryptase in mucosa of children with IBD was 21.77±20.2 ng/g and 18.9±15.98 ng/g in children with no abnormalities on colonoscopy. There is no statistically significant difference between studied and control group. No difference was also found between the groups with 3 forms of IBD: tryptase concentration was 16.18±15.84 ng/g in ulcerative colitis, 28.35±21.61 ng/g in Crohn's disease and 24.95±20.97 ng/g in nonspecific colitis. ConclusionThe tryptase activity in children does not correlate with the presence of inflammation in intestinal mucosa.
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