Abstract
The activity of peroxidase were observed in leave of banana clone Kepok resulting by treatments EMS. The phenotype of peroxidase wasanalyzed from the banana clones resulting the induced by EMS and without induction after inoculated with pathogen Banana Blood deasesBacterium (BBD). The objectives of research are know variation of activity and band pattern of peroxidase. Induced mutation treatmentsconsist of control, 0.2% EMS for 1 and 3 hours, 0.5% for 1 and 3 hours, each treatments was provided five banana clones. The result showedthat variant value and coefisien variant of peroxidase activity in leaf tissue of clone treated by EMS increased compare to the control. Avariant control is 0.28 with coefisien variant is 29.92%, while variant value of treatment is 8.45 with coefisien variant is 75.75%.Appearance of peroxidase bands on clone resulting by EMS treatments were emerged four band pattern. The first and control band patternhas relative migration distance is 20 and 30, the second 15, 40 and 60, the third is 15, 35 and 50. There are four bands with different relativemigration distance that indicated polymorphic.
Highlights
PENDAHULUAN Pisang merupakan salah satu komoditi ekspor yang bernilai penting karena sebagai bahan untuk industri pisang olahan dan sebagai sumber pangan substitusi beras
The activity of peroxidase were observed in leave of banana clone Kepok resulting by treatments
The phenotype of peroxidase was analyzed from the banana clones resulting the induced by
Summary
Semakin meningkatnya koefisien keragaman dan nilai varian menunjukkan bahwa terjadi peningkatan variasi aktivitas enzim peroksidase pada mutan bibit pisang Kepok. Peningkatan variasi ini terjadi akibat telah terjadinya mutasi pada gen penyandi enzim peroksidase. Induksi mutasi dengan mutagen kimia EMS dapat berpengaruh terhadap tingkat perubahan aktivitas enzim peroksidase (Sussiyanti 2000). Data yang ditampilkan pada Tabel 1 menunjukkan bahwa nilai varian dan koefisien keragaman aktivitas enzim peroksidase jaringan daun tanaman kontrol juga cukup tinggi yaitu 29,92%, meskipun perbanyakan secara pisang melalui vegetatif. Ini menunjukkan adanya penambahan keragaman isozim pada enzim peroksidase yang terbentuk sebagai akibat dari perubahan struktur/susunan rantai polipeptida yang membangun molekul protein/enzim peroksidase. Akibat kesalahan ini terjadi perubahan struktur pada susunan basa nukleotida yang juga berpengaruh terhadap sintesis enzim/proteinnya
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