Abstract
<p class="StyleAuthorBold"><em>Pirdot is a natural medicinal plant contains flavonoids that has antibacterial properties. The bacteria tested in this experiment is E. coli that causes of diarrhea. This study was to determine the antibacterial effect of pirdot leaves made in an extract preparation by the diffusion method using paper discs.. This study also used Tetracycline as a comparison, where this antibiotic is very sensitive to E. coli bacteria. Pirdot leaf extract was made in various concentrations, namely 12.5%, 15%, 17.5% and 20% and 20 mcg and 30 mcg of Tetracycline concentrations. The results showed that pirdot leaf extract in E. coli was able to inhibit with a concentration of 12.5% </em><em></em><em>of 10.41 mm, 15% of 11.71 mm, 17.5% of 14.08%, and 20% of 15.43 mm, while the 20 mcg Tetracycline comparison was 18.73 mm and 20.71 mm at 30 mcg. So the conclussion from this research are the concentration of 12.5% and 15% can not be not said to be antibacterial but at concentrations of 17.5% and 20% can be said to be antibacterial. </em><em></em></p><p class="abstrak" align="left"><em>Keywords: Pirdot leaf, Eschericia coli, antibacterial</em>. </p>
Highlights
Pirdot (Saurauia vulcani) adalah jenis tumbuhan liar berada hutan Sumatera
The results showed that pirdot leaf extract in E. coli was able to inhibit with a concentration of 12.5% of 10.41 mm, 15% of 11.71 mm, 17.5% of 14.08%, and 20% of 15.43 mm, while the 20 mcg Tetracycline comparison was 18.73 mm and 20.71 mm at 30 mcg
Identifikasi Golongan Senyawadan Aktivitas Aktioksidan Ekstrak Metanol Serta Fraksi Aktif Daun Pirdot (Saurauia Vulcani)
Summary
Digunakan metode eksperimental yaitu untuk uji aktivitas antibakteri terhadap ekstrak daun pirdot terhadap pertumbuhan bakteri E.coli yang dilakukan di Laboratorium Biologi Universitas Sari Mutiara Indonesia yaitu untuk membuktikan adakah efek yang diberikan ekstrak daun pirdot terhadap baktesi E.coli dan pada konsentrasi yang berbeda. Perhitungan ekstrak daun pirdot yaitu, misalnya simplisia yang ditimbang 10 bagian adalah 600 gram. Daun pirdot yang telah kering ditimbang 600 gram, lalu dimasukkan beaker glass tertutup minimal 3 jam. Cairan akan menetes dengan kecepatan 1 ml per menit, kemudian ditambahkan berulang – ulang cairan penyari hingga selalu terdapat selapis cairan penyari di simplisia, sampai diperoleh 80 bagian perklorat. Setelah sterilangkat dari autoklaf pada suhu 121 °C selama kurang lebih 15 menit. Pengujian efek antibakteri ekstrak daun pirdot dengan mensterilkan alat dan bahan yang akan dipakai. Dicampurkan masing – masing bakteri dengan konsentrasi 106 ke 100 ml NA pada temperature 45 °C – 50 °C, dihomogenkan lalu dituang sebanyak 15 ml ke masing – masing cawan petri dan dibiarkan memadat. Rendam cakram kertas ke ekstrak daun pirdot dengan masing – masing konsentrasi lalu biarkan selama 2 menit. Catat hasilnya dalam milimeter (mm). percobaan ini dilakukan secara triplo (3 cawan petri sekaligus)
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