Abstract
Objective: This work is mainly focused to determine the antibacterial activity of the green synthesized Bi2O3 nanoparticles against the bacterial strains, Staphylococcus aureus and Escherichia coli using resazurin as indicator.
 Methods: Bismuth oxide nanoparticles were synthesized from the precursor bismuth nitrate [Bi (NO3)3.5H20] by using trachyspermum ammi (ajwain) seed extract. To carry out these works, the synthesized Bi2O3 NPs undergone characterizations and were confirmed by UV-Vis, FT-IR, XRD, SEM and EDAX, TGA-DTA and DLS. Biological activity was done using a well diffusion method.
 Results: Bi2O3 NP's has been tested against bacteria (S. aureus and E. coli) in wells and shows blue colour, indicating bacterial growth inhibition in a dose-dependent manner for different concentrations.
 Conclusion: The biological studies were done with one gram-positive and one gram-negative bacteria to show the inhibiting efficiency. The synthesized bismuth oxide nanoparticles showed good anti-bacterial activity (different concentrations) against S. aureus and E. coli.
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