Abstract
The process of ecdysteron extraction from the aerial part of Ajuga turkestanica was studied. On the basis research results established the following extraction conditions: extractant – 95% ethyl alcohol, the particle size of the raw material is 2–5 mm and the process temperature at 20–30 °C. Proposed fivefold ecdysteron extraction from the aerial part of Ajuga turkestanica. To remove hydrophobic impurities from the concentrated and water-diluted extract, proposed was a sequential treatment with extraction benzine and ethyl acetate. From the purified aqueous solution, ecdysteron is extracted with n-butanol. From a concentrated butanol solution, precipitation is recommended of iridoids from acetone. For chromatographic purification, the simultaneous use of aluminum oxide and silica gel in the ratio of the sum of ecdysteroids – sorbent 1 : 20. The quality as eluent mix selected propanol-2 – chloroform in a volumetric ratio of 1 : 3, and for crystallization a mixture of methanol with ethyl acetate in a volume ratio of 1 : 9. The technology has been developed for obtaining the ecdisten substance from the arial part of Ajuga turkestanica.
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