Abstract
Background: Conserved noncoding sequence-1 (CNS-1) is an important regulatory element for T helper 2 cytokine expression. IL-4, IL-5 and IL-13 expression as well as serum IgE level were attenuated in CNS-1<sup>–/–</sup> mice. Method: CNS-1<sup>–/–</sup> and CNS-1<sup>+/+</sup> mice were sensitized with ovalbumin (OVA) followed by antigen challenge. The number of eosinophils and T helper 2 cytokine concentration in the bronchoalveolar lavage fluid, OVA-specific IgE antibody (Ab) in the serum and bronchial responsiveness to methacholine were examined. Results: Bronchoalveolar lavage fluid eosinophilia was significantly attenuated in CNS-1<sup>–/–</sup> mice compared to CNS-1<sup>+/+</sup> mice, which were sensitized with OVA/aluminum once. OVA-specific IgE Ab was also attenuated. When mice were sensitized with OVA/aluminum twice, induction of eosinophilia and OVA-specific IgE Ab was not significantly different between CNS-1<sup>–/–</sup> and CNS-1<sup>+/+</sup> mice. Conclusion: CNS-1 locus regulates eosinophilic inflammation in vivo.
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