Air-drying causes an alteration in density fractionation of soil materials from organic horizons containing volcanic ash

Abstract

Density fractionation is commonly used to isolate organic matter in soils based on organo-mineral associations. Not only mineral horizons but also organic horizons contain mineral components. Soil samples for density fractionation are sometimes air-dried or freeze-dried, which can introduce unexpected biases in the results. In this study, we compared the density fraction distributions in wet, air-dried, and freeze-dried organic horizon samples rich in reactive Al and Fe minerals. On Mt. Chokai, a volcano in northern Japan, samples were collected from organic horizons from three representative vegetation types: K1 (dwarf pine and dwarf bamboo at the summit), K2 (dwarf bamboo on the back slope), and K3 (snow meadow with predominantly Poaceae at the foot slope). Wet, air-dried, and freeze-dried samples were subjected to sequential density fractionation using cutoff values of 1.6 and 1.8 g cm–3. We then determined the mass, organic carbon, and total nitrogen distributions in the isolated fractions. Air-drying altered the mass, organic carbon, and total nitrogen distribution of each density fraction. Although we observed differences in the mass distributions of the freeze-dried and wet samples in the snow meadow samples (K3), the mass distributions in the K1 and K2 samples did not change significantly between the freeze-dried and wet samples. Therefore, freeze-drying is recommended as a pretreatment to minimize the effects of drying on density fractionation, particularly for soil samples with no drying history, high organic matter content, and/or high levels of reactive Al and Fe minerals.