Abstract
The ground nestingTrigona denoiti as well as the arboreal nestingT. gribodoi have nest cavities with only a single narrow entrance tube. For air exchange in the nest to occur, the bees have to ventilate the nest actively. As a consequence the air is exchanged in a similar way as in vertebrates with lungs. Phases of inspiration follow phases of expiration (‘breathing’ frequency in the range of 2–3 breaths/min) and tidal volumes range from 0.63±0.24 ml inT. denoiti to 1.13±0.32 inT. gribodoi. Ventilation during night time was strongly reduced.
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