Abstract
The recombinant antibody AI179 against the Myc tag detects a Myc-tagged protein exogenously expressed in human cells by Western blotting.
Highlights
The Myc tag or c-Myc tag is a ten amino acid long peptide derived from the human protein c-Myc (Munro et al, 1986), which is recognized by the monoclonal antibody 9E10 (Evan et al, 1985)
Cells transfected with an unrelated expression vector were used as a negative control
After 10 min of incubation at room temperature, extracts were centrifuged at 16'100 g for 10 min, and the pellet was discarded
Summary
The Myc tag or c-Myc tag is a ten amino acid long peptide derived from the human protein c-Myc (Munro et al, 1986), which is recognized by the monoclonal antibody 9E10 (Evan et al, 1985). Antibodies: ABCD_AI179 (ABCD nomenclature, https://web.expasy.org/abcd/) targets the Myc tag epitope EQKLISEEDL. The antibody was produced by the Geneva Antibody Facility (https://www.unige.ch/ medecine/antibodies/) as a mini-antibody with the antigenbinding scFv fused to the Fc region of a mouse IgG1.
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