経口および経静脈脂質負荷による血清アポリポ蛋白A-I, A-II濃度の変動

Abstract

Serum apolipoprotein A-I and A-II levels were determined after oral and intravenous fat administration to elucidate the correlation between lipid metabolism and high density lipoprotein. For oral fat administration, four normal adult males ingested 55g of butter after an overnight fast. For intravenous fat administration, five normal adult males and eleven patients with liver disease were injected 1ml of 10% fat emulsion (Intralipid) per kg of body weight after an overnight fast. The A-I and A-II measurement was carried out by the rocket immunoelectrophoresis. Serial blood specimens were taken and measured for their concentration of A-I, A-II and triglyceride.Serum apo A-I and A-II levels scarcely varied after intravenous fat administration, but in some cases decreased slightly. On the other hand, the apoprotein levels increased after oral fat administration and peaked after 60 minutes. Serum apo A-I and A-II levels varied concomitantly, therefore, the ratio of apo A-I/apo A-II remained constant. These results suggested that serum apolipoprotein A-I and A-II levels were influenced partially by their synthesis in the small intestine.