Agronomic treatments to avoid seed presence in ‘Nadorcott’ mandarin I. Effect on in vivo pollen tube growth

Abstract

Fresh market demands high quality fruit and, therefore, citrus growers and researchers are constantly looking for solutions to avoid seed presence. Current horticultural techniques have low effectiveness or high cost. The objective of this study is to evaluate the inhibition effect of seven products on the in vivo pollen tube growth in Nadorcott mandarin, which is a high-value seedy variety. To achieve this main objective, three inorganic fertilizers (ammonium nitrate, potassium nitrate, sulfur), and four saccharides (saccharose, methyl cellulose, callose, chitosan) were applied to Nadorcott stigmas 24 h before and after hand pollination. Pollen tubes were counted 1, 3, and 5 days after treatment in longitudinal blue violet autofluorescence-stained sections. Of the seven evaluated products, only sulfur had a strong inhibitory effect. Elemental sulfur (S8) inhibited pollen tube growth by 94-100%. This strong effect was observed regardless of sulfur being applied 24 h before or after pollination, and on fixed flowers 1, 3 or 5 days after applications. Saccharose treatment seemed to have the opposite effect: stimulated pollen tube growth, but the difference with the positive control was small and non-significant. The sulfur effect could be useful for designing agronomic applications capable of preventing seed presence in Nadorcott mandarin.