Abstract
Various inoculation procedures of in vitro grown wheat seedlings with Agrobacterium tumefaciens carrying a wheat dwarf virus (WDV) dimer led to 13–46% agroinfection. Deliberate wounding of seedlings during or before inoculation gave higher agroinfection rates than in seedlings without wounding. Seedlings from 1–4 days old were equally receptive to inoculations in the meristematic regions. Vacuum infiltration of the Agrobacterium inoculum into excised cultured wheat embryos gave up to 35% agroinfection (with 4 ωg 1 −1 acetosyringone). WDV was detectable in seedlings by the ELISA assay 4 days after inoculation and there was good agreement between the ELISA and DNA dot blot assays of virus infection. Agrobacterium was found to deliver DNA (WDV) to the three wheat varieties tested and to Aegilops speltoides, Triticum monococcum and T. durum.
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