Abstract
The present study investigated the potential lipase production for Bacillus licheniformis SMIA-3 using the agro-industrial co-products: orange flour (OF) and grape flour (GF) blend waste cooking oil (WCO). The OF was selected due to its best source for lipase production observed in preliminary tests. Therefore, OF was tested at different fermentation times at 50°C using the statistical design Central Composite Rotatable Design (CCRD) allied to the response surface. An optimal region was found with lipolytic activity of 0.349 U mL-1 with OF and WCO filters around (0.50% w v-1) and between (0.55 and 0.75% w v-1), respectively, and the fermentation time at the central point (42h). Data supplied a method to produce lipase using orange flour and frying oil, as a way to reuse these waste as feedstock to obtain employable lipase and lower production costs with biotechnological applications in industrial sector
Highlights
This study aims to investigate the potential of production of lipase for Bacillus licheniformis SMIA-3 using agro-industrial co-products and waste cooking oil
The microorganism used in this study was Bacillus licheniformis SMIA-3, kindle provided by the Microbiology Sector of the Food Technology Laboratory of the State University of North Fluminense (UENF)
Influence of the use of orange flour (OF), grape flour (GF) and waste cooking oil (WCO) for lipase production Addition of agro-industrial co-products in medium fermentation was evaluated to verify the potential of the lipase production by thermophilic Bacillus licheniformis SMIA-3 (Table 2)
Summary
Lipases (triacylglycerol acylhydrolases EC 3.1.1.3) are enzymes of the hydrolases family that catalyze the hydrolysis of triacylglycerols in diacylglycerides, monoacylglycerides, free fatty acids and glycerol under aqueous conditions (Mendes, Oliveira, & Castro, 2012; Saraswat, Verma, Sistla, & Bhushan, 2017). In non-aqueous conditions, the enzyme catalyzes the reverse reaction (esterification, interesterification and transesterification) and produces acylglycerols from glycerol and fatty acids (Saxena, Sheoran, Giri, & Davidson, 2003). They are considered to be excellent substances due to their versatility in catalyzing countless reactions, which broadens their application and enables their use in several industrial sectors such as detergents, cosmetics, paper production, food, biodiesel and biopolymers synthesis (Barros, Fleuri, & Macedo, 2010)
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