Abstract
Abstract: The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% – half strength, and 100% - full strength) in the infection and cocultivation media on genetic trans-formation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A. tumefaciens harboring the binary vector pTF102 containing the genes uidA and BAR under control of the CaMV35S promoter. The efficiency of the transgenic events produced was 3% for full strength and 1.1% for half strength N6 salts. Although under a lower concentration of salts, GUS expres-sion was stronger; under this concentration, regeneration was less efficient. Thus, the results showed that the presence of 100% N6 salts in the infection and cocultivation media favored genetic transformation of the L3 maize inbred line mediated by A. tumefaciens. Key words : Immature embryos, salt concentration, somatic embryogenesis, Zea mays.
Highlights
Brazil produced 84.7 million tons of maize on 15.7 million hectares in 2015 (Conab 2016) and it is estimated that 80% of the maize harvested is genetically modified
Governmental and private agricultural institutions in Brazil have been conducting investigations related to the adaptation of maize to biotic and abiotic stresses aiming at an increase in yield, and genetic transformation of tropical maize lines may have a major impact on the development of new products based on this knowledge
Maize embryogenic cultures have two predominant forms of callus, Type I and Type II, which differ primarily in their regeneration efficiency over an extended time in a culture medium. Both calli are capable of plant regeneration, cultures formed by the Type II callus grow faster; can be maintained for a longer period of time, and form a larger
Summary
Brazil produced 84.7 million tons of maize on 15.7 million hectares in 2015 (Conab 2016) and it is estimated that 80% of the maize harvested is genetically modified. Governmental and private agricultural institutions in Brazil have been conducting investigations related to the adaptation of maize to biotic and abiotic stresses aiming at an increase in yield, and genetic transformation of tropical maize lines may have a major impact on the development of new products based on this knowledge. Maize embryogenic cultures have two predominant forms of callus, Type I and Type II, which differ primarily in their regeneration efficiency over an extended time in a culture medium. Both calli are capable of plant regeneration, cultures formed by the Type II callus grow faster; can be maintained for a longer period of time, and form a larger
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