Abstract
Genetic transformation is a versatile platform that is playing increasingly important roles in functional genomics studies and crop improvement. Cucumber (Cucumis sativus L.) is an economically important vegetable crop, and a model of choice to understand several biological processes. Although many genetic transformation studies have been carried out in the last four decades, the transformation efficiency and reproducibility of cucumber transformation remain low limiting its widespread and routine use. In this chapter, we reviewed the work in cucumber on Agrobacterium tumefaciens-mediated genetic transformation, and discussed factors affecting its efficiency in each step including genotypes, explant types, the time, temperature and bacterial concentration for co-cultivation, as well as growth regulators and selective agents for transgenic plant regeneration. We emphasized the importance to develop an efficient, reliable and reproducible cucumber genetic transformation system through collaborative and community efforts.
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