Abstract
Agrobacterium Mediated Transformation of Tobacco BY2 Cell Suspension with Two Different Zinc Transporters
Highlights
A small number of plant species has metal hyperaccumulating properties
Some of the filter papers were transferred to selective plates with different Zincconcentration
Callus growth was observed on only four plates, including both negative control plates and the non-selective plates of the BG0014 and BG0073 transformation
Summary
A small number of plant species has metal hyperaccumulating properties. These plants accumulate metals to a very high level in their shoots, without suffering from toxicity effects (Hassan and Aarts., 2011; Verbruggen et al, 2009). The N. caerulescens ZNT1 gene shows highest homology to Arabidopsis ZIP4 gene, which was discovered by Grotz et al in 1998 It was further researched and characterized by Hassan (2013), who confirmed the localization of the ZNT1 gene in the plasma membrane while characterized it as a functional Zn transporter protein. Transport of zinc into the vacuole is an effective way to detoxify the ions For this reason, AtMTP1 mutants show a hypersensitive reaction to zinc, which confirms the essential role of the AtMTP1 protein in zinc accumulation and detoxification. AtMTP1 mutants show a hypersensitive reaction to zinc, which confirms the essential role of the AtMTP1 protein in zinc accumulation and detoxification These findings are further underlined by the results of Hassan., 2013, who concluded that introduction of the ZNT1 or the MTP1 gene in Arabidopsis confers tolerance to zinc stress. This property makes both zinc transporters very interesting topics for further research
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