Agrobacterium-mediated transformation of peach (Prunus persica L. batsch) leaf segments, immature embryos, and long-term embryogenic callus

Abstract

Peach leaf segments, immature embryos, and long-term embryogenic calli have been transformedin vitro with the engineeredAgrobacterium tumefaciens strain A281 containing pGA472. All three tissue sources proliferated callus which grew on a medium containing 100–200 mg/l kanamycin or 10–20 mg/l G-418 as selective agents. These calli were shown to produce neomycin phosphotransferase. The results of Southern analyses were consistent with the incorporation of foreign DNA into the genome of leaf, embryo and embryogenic peach callus.