Abstract
Lettuce cultivars. Diana and Cobham Green were used for transformation using the binary vector system of Agrobacterİum tumefaciens. A \beta-1, 3-glucanase gene from Arthrobacter driven by the 35 S prometer and NPT 11 gene expressed from Nos promoter were involved as scorible and selectable genes. respectively. Transformations were obtained at a high frequency particulary with cultivar Cobham Green (80%). Transformation was confirmed by using Southern and Northern blot analysis. NPT 11 enzyme assay and segregation of resistance to kanamycin. Our results showed that this protocol can be efficiently used for transforming Iettuce.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
