Abstract
Agrobacterium tumefaciens causes crown gall disease, which comprises disorganized proliferating tumor cells on many gymnosperm and dicotyledonous angiosperm plants. This chapter concentrates on Agrobacterium-mediated plant transformation. The DNA that is placed between the 25-bp border sequences in Agrobacterium retaining a full complement of vir functions can be efficiently transferred and stably integrated into the plant genome. This property provides the basis for producing Ti plasmid vector systems for plant cell transformation. The host of Agrobacterium for plant transformation contains a helper Ti plasmid deleted of its T-DNA and provides the necessary vir functions to transfer the DNA sequences between the 25-bp border repeats in the separate replicon. The chapter describes useful methods for the transformation of plant cells. It also describes dominant selectable markers and negative selection markers, and the assayble markers. Negative selection markers have been developed that are lethal or interfere with normal development of the plant.
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