Abstract
In recent years, researchers have highlighted the role of low cost-efficient agro-industrial by-products used as supplements in algal culture media. The aim of the study was to identify and characterize the basic metabolic pathways in Tetradesmus obliquus cells induced by supplementation with beet molasses in photoheterotrophic and mixotrophic culture conditions. To assess the impact of the nutritional strategy in unicellular algae, growth curves were plotted and lipid, carbohydrate, and protein levels were determined. Fourier Transform Infrared Spectroscopy was applied to measure the Tetradesmus obliquus cell composition. Additionally, the C16-C18 fatty acid profile of Tetradesmus obliquus was determined by gas chromatograph/mass spectrometry. The switch from autotrophy to photoheterotrophy and mixotrophy contributes to shortening of the adaptation growth phase. The highest protein content was obtained in the mixotrophic growth. This study has demonstrated high content of 18:1, cisΔ9, 18:2, cisΔ9,12, ω6, and 18:3, cisΔ9,12,15, ω3 in photoheterotrophic and mixotrophic culture conditions. High levels of proteins and essential fatty acids make Tetradesmus obliquus cell biomass important for human and animals health.
Highlights
In recent years, researchers have highlighted the role of low cost-efficient agro-industrial by-products used as supplements in algal culture media
The aim of this study is to identify and characterize the basic metabolic pathways in unicellular green algae T. obliquus induced by supplementation with beet molasses as a source of carbon and nitrogen
The short adaptation phase in the photoheterotrophic and mixotrophic culture conditions may result from the presence of organic carbon compounds derived from beet molasses in the culture medium
Summary
Researchers have highlighted the role of low cost-efficient agro-industrial by-products used as supplements in algal culture media. The aim of the study was to identify and characterize the basic metabolic pathways in Tetradesmus obliquus cells induced by supplementation with beet molasses in photoheterotrophic and mixotrophic culture conditions. High levels of proteins and essential fatty acids make Tetradesmus obliquus cell biomass important for human and animals health. Tetradesmus obliquus (Scenedesmus obliquus) is considered as a suitable candidate for utilization of organic carbon sources of agricultural origin in mixotrophic culture conditions, especially in biodiesel production[17]. The aim of this study is to identify and characterize the basic metabolic pathways in unicellular green algae T. obliquus induced by supplementation with beet molasses as a source of carbon and nitrogen. The study investigates the effect of autotrophic, photoheterotrophic, and mixotrophic culture conditions on growth parameters and biochemical composition with special consideration of the C16–18 fatty acid profile in T. obliquus cells
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
