AGREE, hexagonal and whiteness assessment approaches for evaluating two novel analytical methods; capillary zone electrophoresis and spectrophotometric assays for simultaneous determination of pantoprazole, chlordiazepoxide, and clidinium bromide ternary mixtures

Abstract

Going Green through pharmaceutical analysis is not a luxury anymore. Maintaining sustainability and eco-friendliness is targeted as urgently as the validation of the newly developed procedures is. Although no firm guidelines have been implemented yet, the AGREE, Hexagon, and Whiteness “RGB 12” models confirm and fairly arbitrate the method greenness approval. In this consensus, two novel methods; Capillary Zone Electrophoresis-Diode Array Detection and Double Divisor Ratio Spectra Derivative Spectrophotometric assays are developed and fully validated for the simultaneous determination of Clidinium bromide, Chlordiazepoxide, and Pantoprazole ternary polypill. Linearity ranges of 1–100 μg/mL for the three drugs were achieved using CZE, meanwhile, 2.5–50, 1–30, and 5–80 μg/mL for Clidinium bromide, Chlordiazepoxide, and Pantoprazole, respectively were achieved using Spectrophotometry. Both methods have been validated as per International Council for Harmonization guidelines and applied for three drugs’ estimation in laboratory-made pills. Greenness and sustainability appraisals of the proposed procedures were evaluated by the three former models versus the reported HPLC and HPTLC methods for the cited mixture. Suggested methods declared higher green attributes than previous ones.