Agranulocyte & neutrophil nucleus enhancement through 510nm wavelength of light

Abstract

Enhanced features can be easily segmented and used for classification algorithms. Light wavelength of 510nm is used as illumination source for bright field microscope. This specific wavelength of light enhances nucleus, granules in White blood Cells (WBCs). Eosinophil and basophil has coarse granules which also get enhanced hence nucleus segmentation gets difficult. This method is effective in enhancing nucleus of agranulocytes viz. Lymphocyte, Monocyte and Neutrophil of normal person. This modification in image acquisition of pathological images will further help in quick segmentation of features.